| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T17 |
0-916 |
Sentence |
denotes |
Proteomics is an effective tool for the comprehensive analysis of host cellular responses to viral infections, which is conducive to elucidating the underlying pathogenesis of the virus.18 The currently available proteomics techniques include two-dimensional gel electrophoresis,15 two-dimensional difference gel electrophoresis,19 stable isotope labeling by amino acids in cell culture,20,21 isobaric tags for relative and absolute quantitation (iTRAQ),22 and label-free proteomic techniques.23 Among all these mentioned techniques, iTRAQ coupled with liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis has shown several comparative advantages over its counterparts, for instance, high sensitivity, high throughput, high separating capacity, and high accuracy, and thus emerged as a robust quantitative proteomics technique for the comprehensive analysis of differentially expressed proteins (DEPs). |
| T18 |
917-1367 |
Sentence |
denotes |
To date, this technique has been successfully applied to numerous studies involved in virus-host interactions, examples of which include TGEV,22 PEDV,24 foot-and-mouth disease virus (FMDV),25 porcine circovirus type 2 (PCV2), and classical swine fever virus (CSFV).26 These studies have given a good overview of the dynamic interactions between the virus and its host, and provide important clues for a better understanding of the viral pathogenesis. |
| T19 |
1368-1433 |
Sentence |
denotes |
For PDCoV, there has been no proteomic study on the virus so far. |
| T20 |
1434-1570 |
Sentence |
denotes |
In the present study, we coupled iTRAQ with LC-MS/MS to quantitatively analyze the DEPs of IPEC-J2 cells in response to PDCoV infection. |
| T21 |
1571-1659 |
Sentence |
denotes |
The identified DEPs were subsequently analyzed by comprehensive bioinformatics analyses. |
