LitCovid-sentences  

PMC:7640975 / 34431-36077 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
T233 0-178 Sentence denotes To validate the obtained LC-MS/MS data, qPCR was performed to evaluate the transcription levels of two randomly selected DEPs, the downregulated ANAPC7 and the upregulated IFIT1.
T234 179-265 Sentence denotes To this end, IPEC-J2 cells were mock-infected or infected with PDCoV at an MOI of 0.1.
T235 266-354 Sentence denotes At 24 hpi, total cellular RNA was extracted from the cells and subjected to qPCR assays.
T236 355-568 Sentence denotes As shown in Figure 2A, the level of mRNA encoding ANAPC7 and IFIT1 proteins was significantly downregulated and upregulated in PDCoV-infected cells, respectively, as compared to the mock-infected cells (p < 0.05).
T237 569-675 Sentence denotes The qPCR results were in agreement with the MS data which were acquired by the iTRAQ approach (Figure 2B).
T238 676-895 Sentence denotes For further confirmation of the proteomic data, the expression level of ANAPC7 and IFIT1 proteins in IPEC-J2 cells, which were infected exactly as the aforementioned conditions, was also tested by Western blot analysis.
T239 896-1001 Sentence denotes To track the progression of PDCoV infection, the mAb 1A3 that specifically recognizes PDCoV was utilized.
T240 1002-1335 Sentence denotes As shown in Figure 2C, compared with the mock-infected IPEC-J2 cells, PDCoV significantly decreased the expression of ANAPC7 protein and its relative ratio to β-actin in the cells, whereas the expression of IFIT1 protein and its relative ratio to β-actin in the cells were significantly increased as a consequence of PDCoV infection.
T241 1336-1462 Sentence denotes The original images of the entire PVDF membranes containing the target Western blots were included in Supplementary Figure S2.
T242 1463-1538 Sentence denotes The Western blot results were also consistent with the MS data (Figure 2D).
T243 1539-1646 Sentence denotes Taken together, these experimental results reveal that our quantitative proteomics data are quite reliable.