Id |
Subject |
Object |
Predicate |
Lexical cue |
T122 |
0-247 |
Sentence |
denotes |
To obtain the optimal inoculation dose that could produce DEPs to the greatest extent, IPEC-J2 cells were inoculated with PDCoV at low, medium, and high MOIs (i.e., 0.01, 0.1, and 1 TCID50/cell, respectively) for 36 h before the formal experiment. |
T123 |
248-428 |
Sentence |
denotes |
By observing the CPE, we found that the high MOI resulted in a rapid synchronous infection of all the cells, causing majority of the cells detached and disintegrated within 12 hpi. |
T124 |
429-502 |
Sentence |
denotes |
This is not conducive to the interaction between the virus and the cells. |
T125 |
503-687 |
Sentence |
denotes |
By contrast, the cells inoculated with the low MOI only displayed minimal CPEs at the same time points, which might induce limited changes in the expression levels of diverse proteins. |
T126 |
688-806 |
Sentence |
denotes |
Therefore, the medium MOI of 0.1 was selected as the optimal dose for PDCoV inoculation in all subsequent experiments. |