Id |
Subject |
Object |
Predicate |
Lexical cue |
T4 |
69-174 |
Sentence |
denotes |
Porcine small intestinal epithelial cells (IPEC) are the primary target cells of PDCoV infection in vivo. |
T5 |
175-431 |
Sentence |
denotes |
Here, isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled to liquid chromatography–tandem mass spectrometry (LC-MS/MS) was used to quantitatively identify differentially expressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. |
T6 |
432-546 |
Sentence |
denotes |
A total of 78 DEPs, including 23 upregulated and 55 downregulated proteins, were identified at 24 h postinfection. |
T7 |
547-616 |
Sentence |
denotes |
The data are available via ProteomeXchange with identifier PXD019975. |
T8 |
617-972 |
Sentence |
denotes |
To ensure reliability of the proteomics data, two randomly selected DEPs, the downregulated anaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), were verified by real-time PCR and Western blot, and the results of which indicate that the proteomics data were reliable and valid. |