LitCovid-sentences  

PMC:7640975 / 17368-19151 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T91 0-38 Sentence denotes 2.9 Quantitative Real-Time PCR (qPCR)
T92 39-219 Sentence denotes To verify the DEPs identified by iTRAQ at the transcriptional level, the mRNA of two representative DEPs, the downregulated ANAPC7 and the upregulated IFIT1, were detected by qPCR.
T93 220-371 Sentence denotes Total cellular RNA of both PDCoV- and mock-infected IPEC-J2 cells was extracted using the TaKaRa MiniBEST universal RNA extraction kit (Dalian, China).
T94 372-552 Sentence denotes The first strand cDNA was synthesized using 2 μg of cellular RNA and with a RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) following the manufacturer’s instructions.
T95 553-701 Sentence denotes The qPCR assays were conducted using a TaKaRa SYBR Premix Ex Taq kit on an ABI 7500 real-time PCR machine along with β-actin as a housekeeping gene.
T96 702-906 Sentence denotes The primers used for the qPCR assay were designed based on the NCBI reference sequences for ANAPC7, IFIT1 and β-actin genes under GenBank accession numbers NM_016238, HQ679904 and NM_001101, respectively.
T97 907-982 Sentence denotes The information for the designed primers was as following: ANAPC7 (Forward:
T98 983-1019 Sentence denotes 5′-CTTTGCTGAGGAACGCACTG-3′, Reverse:
T99 1020-1064 Sentence denotes 5′-TCCATGTCGTCCACATCCTC-3′); IFIT1 (Forward:
T100 1065-1106 Sentence denotes 5′-GAAGATTTAACCCAACAAGAACATA-3′, Reverse:
T101 1107-1158 Sentence denotes 5′-CTTTCGATACGTAAGGTAATACAGC-3′); β-actin (Forward:
T102 1159-1195 Sentence denotes 5′-TCCCTGGAGAAGAGCTACGA-3′, Reverse:
T103 1196-1224 Sentence denotes 5′-AGCACTGTGTTGGCGTACAG-3′).
T104 1225-1434 Sentence denotes The qPCR assays were carried out in a 20-μL reaction volume comprised 10 μL of TaKaRa SYBR Premix Ex Taq, 0.4 μL of each forward and reverse primer (10 μM), 7.2 μL of nuclease-free water, and 2 μL of template.
T105 1435-1671 Sentence denotes The qPCR amplifications were carried out using a thermal profile with an initial step at 95 °C for 2 min, followed by 40 cycles of 95 °C for 10 s, 60 °C for 10 s (for ANAPC7 and β-actin) or 55 °C for 10 s (for IFIT1) and 72 °C for 40 s.
T106 1672-1783 Sentence denotes Fold-change values were calculated in term of the 2–ΔΔCt method and normalized to the β-actin reference gene.33