| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T614 |
0-263 |
Sentence |
denotes |
A third method called structural information using Overhauser effects and selective labeling (SOS-NMR), relies of STD experiments performed on ligand complexes with different protein samples that have been fully deuterated excluding a specific type of amino acid. |
| T615 |
264-506 |
Sentence |
denotes |
In other words, the data obtained by SOS-NMR gives insight into the ligand-binding amino acid composition and when taken into consideration the 3D structure of targeted protein can be used to establish the structure of protein-ligand complex. |
| T616 |
507-684 |
Sentence |
denotes |
This approach has been demonstrated using two complexes—FKBP complexed to 2-(3′-pyridyl)-benzimidazole and MurA complexed to uridine diphosphateN-acetylglucosamine (UDP-GlcNAc). |
| T617 |
685-766 |
Sentence |
denotes |
The results showed that for FKBP and MurA, only four and three amino acids (FKBP: |
| T618 |
767-792 |
Sentence |
denotes |
Ile, Val, Leu, Met; MurA: |
| T619 |
793-911 |
Sentence |
denotes |
Trp, Phe, His) were needed to be selectively protonated in perdeuterated samples to establish the ligand-binding site. |
| T620 |
912-1024 |
Sentence |
denotes |
Additionally, on average only 6 amino acids were required for accurate identification of ligand-binding surface. |
| T621 |
1025-1127 |
Sentence |
denotes |
According to authors SOS-NMR can greatly improve the early stages of the drug discovery process [397]. |
| T622 |
1128-1255 |
Sentence |
denotes |
Moreover, combining SOS-NMR with other methods can even further increase chances for a positive outcome of an experiment [398]. |
