| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T320 |
0-124 |
Sentence |
denotes |
STD NMR is an application of NOE used to probe the binding of ligands to a specific site within the targeted proteins [256]. |
| T321 |
125-205 |
Sentence |
denotes |
A generic example of detecting ligand binding via STD is presented in Figure 9a. |
| T322 |
206-521 |
Sentence |
denotes |
The STD NMR method follows the same concepts as a normal NOE experiment: a spectrum of the ligand in the free, non-binding form is recorded, the ligand is allowed to bind to the protein, which has a functional group of interest (i.e., methyls) with a saturated signal from a previous selective radiofrequency pulse. |
| T323 |
522-726 |
Sentence |
denotes |
The saturated signal travels to the ligand, increasing the intensity of a signal on the ligand spectrum and finally a difference spectrum is used to determine precisely which sections of the ligands bind. |
| T324 |
727-806 |
Sentence |
denotes |
The difference in peak intensities proves the presence of ligand binding [260]. |
