LitCovid-sentences  

PMC:7594251 / 26944-28862 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
T188 0-6 Sentence denotes 2.2.3.
T189 7-21 Sentence denotes 2D 1H,13C-HSQC
T190 22-258 Sentence denotes 2D- Heteronuclear Single Quantum Coherence (HSQC) experiments are commonly used to help resolve spectral overlap [139] while providing 13C information without the inherent sensitivity losses involved in 13C direct detection (see below).
T191 259-355 Sentence denotes HSQC shows the correlations between directly coupled nuclei [140], e.g., 1H-13C or 1H-15N [140].
T192 356-502 Sentence denotes As such, an HSQC spectrum will show clean peaks for each unique proton directly connected to the heteronuclear nuclear atom of interest [140,141].
T193 503-674 Sentence denotes In 1H,13C/15N HSQC experiments, the magnetization is transferred from the more sensitive nucleus (I:1H) to the less sensitive nucleus (S:13C/15N) [142,143,144] (Figure 3).
T194 675-864 Sentence denotes This is especially useful when applying NMR spectroscopy to drug design, as most drugs are organic (i.e., contain carbon atoms), and the relative abundance of 13C (1.1%) is quite low [143].
T195 865-993 Sentence denotes By transferring sensitivity from 1H to 13C, one can circumvent the long experimental time required for 1D 13C experiments [143].
T196 994-1150 Sentence denotes For example, De Castro et al. [145] studied Ptac2S and its related cytotoxicity to the cisplatin-resistant epithelial ovarian carcinoma (EOC), Skov-3 cells.
T197 1151-1292 Sentence denotes In the study, they used NMR spectroscopy and multi-variate statistical analysis to observe how Skov-3 cells reacted to treatment with Ptac2S.
T198 1293-1521 Sentence denotes In particular, they used 1H,13C-HSQC along with 1H-COSY and Heteronuclear Multiple Bond Correlation (HMBC), and the Human Metabolome Database to assign the chemical shifts of the lipid metabolites present in the studied samples.
T199 1522-1634 Sentence denotes Interestingly, Skov-3 cells treated with Ptac2S produced more pyruvate than Skov-3 cells treated with cisplatin.
T200 1635-1794 Sentence denotes The authors also noticed an unexpected difference in lipid metabolite expression levels between the cells treated with Ptac2S and those treated with cisplatin.
T201 1795-1918 Sentence denotes These results provide a possible explanation for how Ptac2S is able to overcome cisplatin resistance in Skov-3 cells [145].