| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T135 |
0-211 |
Sentence |
denotes |
With a natural abundance of 100% and a gyromagnetic ratio of about 2.5 times smaller than 1H, one may think that phosphorus could be broadly used for NMR experiments regarding the drug discovery and development. |
| T136 |
212-350 |
Sentence |
denotes |
However, the application of 31P is limited due to the fact that most of the molecules of interest simply do not contain a phosphorus atom. |
| T137 |
351-514 |
Sentence |
denotes |
Therefore 31P-NMR is usually applicable for studies related to energy, phospholipid metabolism (ATP, NADP), and/or characterization of changes in DNA [94,119,120]. |
| T138 |
515-693 |
Sentence |
denotes |
For example, Overall et al. conducted an experiment in which they showed that 31P solid-state NMR can be used for quantitative analysis of DNA dynamics within live bacteria [94]. |
| T139 |
694-855 |
Sentence |
denotes |
For that, the researchers first prepared untreated cultures of E. coli, and measured them using a Hartmann-Hahn 1H to 31P cross-polarization (31P CP) experiment. |
| T140 |
856-961 |
Sentence |
denotes |
Afterwards, they measured E. coli treated with ampicillin and maculatin 1.1 (Mac1.1) in a similar manner. |
| T141 |
962-1266 |
Sentence |
denotes |
Spectra obtained from treated bacteria compared to those obtained from untreated bacteria showed alterations in the lineshape, reduced signal intensity at the spectrum’s edges, and a shift in spectral density towards 0 ppm which indicated the increased dynamics of the phosphorus from nucleic acids [94]. |
