| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T347 |
0-83 |
Sentence |
denotes |
Liquid handling using 96-well plates and precautions taken to prevent contamination |
| T348 |
84-150 |
Sentence |
denotes |
To prevent cross-contamination, we have taken several precautions. |
| T349 |
151-257 |
Sentence |
denotes |
The 10× primer mix was prepared with nuclease-free water (AM9937, Ambion) and stored in aliquots at −20°C. |
| T350 |
258-426 |
Sentence |
denotes |
To set up an RT-LAMP test, the RT-LAMP master mix was prepared freshly immediately before the test at a separate workspace with a dedicated pipette set and filter tips. |
| T351 |
427-547 |
Sentence |
denotes |
The 96-well PCR plate containing the RT-LAMP mix was covered with an Society for Biomolecular Screening (SBS) plate lid. |
| T352 |
548-693 |
Sentence |
denotes |
To avoid mix-ups during sample addition through well-by-well pipetting, the RNA or swab specimens were first collected into a 96-well seed plate. |
| T353 |
694-865 |
Sentence |
denotes |
The RNA was then added to the plate with the LAMP reagents at a dedicated workspace with a manual 96-channel pipettor (Liquidator 20 μl, Mettler Toledo) using filter tips. |
| T354 |
866-1085 |
Sentence |
denotes |
The RT-LAMP and the RNA seed plate were instantly sealed with an optically clear adhesive seal (GK480-OS, Kisker Biotech) and an adhesive aluminum foil seal (SL-AM0550, Steinbrenner Laborsysteme, Germany), respectively. |
| T355 |
1086-1300 |
Sentence |
denotes |
If the product of an RT-LAMP reaction had to be analyzed by gel electrophoresis, the plate was opened with extreme caution at a separated post-LAMP workspace and loaded onto an agarose gel with a dedicated pipette. |
