| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T343 |
0-42 |
Sentence |
denotes |
RT-LAMP primer design and positive control |
| T344 |
43-258 |
Sentence |
denotes |
The RT-LAMP primer sets used in this study have been designed by Zhang et al. (11) against ORF1a and N gene and were synthesized by Sigma-Aldrich (synthesis scale, 0.025 μmol; purification, desalt; solution, water). |
| T345 |
259-394 |
Sentence |
denotes |
The sequences and the concentrations of each oligonucleotide in the 10× primer mix used for the RT-LAMP assay can be found in table S1. |
| T346 |
395-768 |
Sentence |
denotes |
An RNA-positive control for the N gene was amplified from a short fragment from 2019-nCoV_N_Positive control plasmid [Integrated DNA Technologies (IDT), 10006625] with oligonucleotides T7-GeneN-Fragment.for and GeneN-Fragment.rev including the T7 promoter and a subsequent IVT with the MEGAscript T7 Kit (Invitrogen) purified using the RNeasy MinElute Cleanup Kit (QIAGEN). |
