| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T320 |
0-253 |
Sentence |
denotes |
Our study was designed to investigate the sensitivity and specificity of a colorimetric RT-LAMP assay and to evaluate its suitability as an alternative to RT-qPCR testing for detecting SARS-CoV-2 viral RNA in RNA isolated from pharyngeal swab specimens. |
| T321 |
254-329 |
Sentence |
denotes |
This study was conducted in Heidelberg, Germany in March and April of 2020. |
| T322 |
330-608 |
Sentence |
denotes |
The study was designed to first evaluate different existing primer sets for RT-LAMP reactions and to use them for (i) detection of SARS-CoV-2 RNA in RNA isolated from pharyngeal swabs and (ii) detection of SARS-CoV-2 RNA directly from swab specimens without prior RNA isolation. |
| T323 |
609-883 |
Sentence |
denotes |
All RNA samples used were pseudo-anonymized surplus material from the Heidelberg University Hospital diagnostic laboratory, and RT-qPCR results for these RNA samples were retrieved from the laboratory’s database only after the samples had been analyzed by the RT-LAMP assay. |
| T324 |
884-1033 |
Sentence |
denotes |
The study design was to conduct RT-LAMP testing until sufficient samples (at least several hundreds) had been analyzed to obtain a conclusive result. |
| T325 |
1034-1213 |
Sentence |
denotes |
We also designed a deep sequencing-based method to validate the outcome of the RT-LAMP reactions using a Tn5 transposase–based fully scalable barcoding strategy (LAMP-sequencing). |
