| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T285 |
0-107 |
Sentence |
denotes |
With its good sensitivity for samples up to CT ≈ 30, the colorimetric RT-LAMP assay has several advantages: |
| T286 |
108-179 |
Sentence |
denotes |
It is fast, inexpensive, and it can be evaluated without any equipment. |
| T287 |
180-428 |
Sentence |
denotes |
RT-LAMP reactions also appear to be less sensitive to contaminants in the samples than RT-qPCR, but care has to be taken that the samples used do not alter the pH as the colorimetric RT-LAMP assay is performed under conditions of weak pH buffering. |
| T288 |
429-593 |
Sentence |
denotes |
Some clinical samples contain contaminants that can lead to acidification of the reaction independent of the presence of a template RNA if too much sample is added. |
| T289 |
594-871 |
Sentence |
denotes |
Diagnostic RT-qPCR tests usually include a technical internal control, i.e., another RNA species, which is spiked into all samples and which is detected independent of the gene of interest to safeguard against the possibility of a general reaction failure within a sample tube. |
| T290 |
872-945 |
Sentence |
denotes |
It would be desirable to have a similar precaution for the RT-LAMP assay. |
| T291 |
946-1074 |
Sentence |
denotes |
A multiplexed fluorescence readout might provide this (34) but comes at the expense of the simplicity of a colorimetric readout. |
