| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T276 |
0-68 |
Sentence |
denotes |
There are several differences between the RT-LAMP assay and RT-qPCR. |
| T277 |
69-298 |
Sentence |
denotes |
First, RT-qPCR requires a thermocycler to conduct the DNA amplification reaction, which is an expensive instrument, whereas isothermal incubation of RT-LAMP reactions can be conducted using a simple water bath or a heating block. |
| T278 |
299-373 |
Sentence |
denotes |
This makes the RT-LAMP assay more amenable for point-of-care applications. |
| T279 |
374-491 |
Sentence |
denotes |
Second, the reagents for the RT-LAMP assay are different from the ones used for RT-qPCR and are supplier independent. |
| T280 |
492-662 |
Sentence |
denotes |
According to the supplier of the RT-LAMP reagents used in this study (New England Biolabs), production of RT-LAMP reagents can be easily ramped up to satisfy high demand. |
| T281 |
663-837 |
Sentence |
denotes |
Third, the RT-LAMP assay, when combined with LAMP-sequencing, is suitable for analyzing large numbers of RT-LAMP reactions owing to the fully scalable DNA barcoding strategy. |
| T282 |
838-975 |
Sentence |
denotes |
In contrast, there are several hurdles to scaling up RT-qPCR assays, the major hurdle being the need for a large number of thermocyclers. |
| T283 |
976-1079 |
Sentence |
denotes |
The RT-LAMP assay overcomes this problem and therefore will be a more scalable method for mass testing. |
