LitCovid-sentences  

PMC:7574920 / 30183-32589 JSONTXT 6 Projects

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Id Subject Object Predicate Lexical cue
T231 0-57 Sentence denotes Heterogeneity of specimen pH in the swab–to–RT-LAMP assay
T232 58-268 Sentence denotes Comparison of the results of the direct swab–to–RT-LAMP assay with the RT-LAMP assay using isolated RNA revealed a much broader distribution of the ΔOD measurements in negative samples (Fig. 5A versus Fig. 3A).
T233 269-375 Sentence denotes This was likely due to a sample-specific variability that influenced the starting pH in the LAMP reaction.
T234 376-462 Sentence denotes This might have affected the interpretability of the measurement at 30 min (ΔOD30min).
T235 463-526 Sentence denotes We investigated how this pH shift influenced the RT-LAMP assay.
T236 527-659 Sentence denotes For three plates, the data acquired for the RT-LAMP assay also included measurements for the 10-min time point (ΔOD10min) (Fig. 6A).
T237 660-842 Sentence denotes We plotted the change of the ΔOD between the 10- and 30-min time points (i.e., the difference ΔOD30min – ΔOD10min, corresponding to the slope of the lines) versus ΔOD30min (Fig. 6B).
T238 843-1025 Sentence denotes This removed the variability of the values for samples that did not change their color (negative samples) and permitted a better separation of the positive from the negative samples.
T239 1026-1095 Sentence denotes Fig. 6 Colorimetric readouts of the swab–to–RT-LAMP assay over time.
T240 1096-1220 Sentence denotes (A) The colorimetric readouts (ΔOD) for the direct (left) and hot (right) swab–to–RT-LAMP assays were assessed every 10 min.
T241 1221-1358 Sentence denotes Heterogeneity is notable at the early time points. ΔOD values at the zero time point were not measured for the hot swab–to–RT-LAMP assay.
T242 1359-1419 Sentence denotes Also, the 40-min time point was not available for one plate.
T243 1420-1545 Sentence denotes The kink in some lines at 30 min (right) was due to a transient equipment malfunction. (B) Comparison of two scoring schemes.
T244 1546-1847 Sentence denotes The readout used in Fig. 5 to score the direct (left) and hot (right) swab–to–RT-LAMP assays, namely, ΔOD at 30 min, is shown on the y axis, and compared to an alternative score, namely, the difference between the ΔOD signals at 30 min and at 10 min after the start of incubation, shown on the x axis.
T245 1848-1921 Sentence denotes The latter shows better separation between positive and negative samples.
T246 1922-2067 Sentence denotes We noticed that the pH variability depended on the sample volume used for the RT-LAMP assay and the composition of the medium used for the swabs.
T247 2068-2233 Sentence denotes For swabs in Amies medium (which was used for the clinical samples in this study), an RT-LAMP assay containing 1 μl of sample in a total volume of 20 μl was optimal.
T248 2234-2406 Sentence denotes Our results obtained using native and heat-treated swab specimens suggested better performance when using heat treatment of swab specimens before running the RT-LAMP assay.