| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T188 |
0-45 |
Sentence |
denotes |
A swab–to–RT-LAMP assay without RNA isolation |
| T189 |
46-161 |
Sentence |
denotes |
RNA isolation is time consuming, costly, and depends on reagents with potentially limited supply during a pandemic. |
| T190 |
162-375 |
Sentence |
denotes |
Alternative, noncommercial solutions for RNA isolation, e.g., using silica gel matrix or magnetic beads, require specialized knowledge and cannot be implemented easily for point-of-care or decentralized screening. |
| T191 |
376-594 |
Sentence |
denotes |
Several reports have indicated that RT-qPCR (18–20) and RT-LAMP assays (21, 22) are compatible with direct testing of nasopharyngeal and oropharyngeal swab specimens without a prior RNA purification or extraction step. |
| T192 |
595-787 |
Sentence |
denotes |
To establish an RT-LAMP assay that could test unprocessed specimens (swab–to–RT-LAMP assay), we first assessed the stability of naked RNA in swab specimens that were collected in Amies medium. |
| T193 |
788-920 |
Sentence |
denotes |
We titrated defined numbers of IVT RNA molecules of the SARS-CoV-2 N gene into swab samples from COVID-19–negative control subjects. |
| T194 |
921-1117 |
Sentence |
denotes |
We tested different conditions, particularly the influence of detergent (to inactivate the virus) and heat (to denature the capsid and release the viral RNA as well as inactivate the virus) (figs. |
| T195 |
1118-1147 |
Sentence |
denotes |
S6 and S7, and data file S1). |
| T196 |
1148-1476 |
Sentence |
denotes |
Consistent with previous reports about other RNA viruses (23–25) and tests using heat inactivation of swab specimens for direct RT-qPCR assays (26), these experiments established that native swab specimens and heat-treated swab specimens were compatible for detection of SARS-CoV-2 RNA in swab samples from infected individuals. |
