PMC:7574920 / 250-1212 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3 0-44 Sentence denotes A colorful test for SARS-CoV-2 RNA detection
T4 45-154 Sentence denotes We need simple methods to rapidly test large numbers of people for infection with the SARS-CoV-2 coronavirus.
T5 155-291 Sentence denotes Quantitative PCR (qPCR) after reverse transcription (RT), the standard method, is very sensitive but requires expensive instrumentation.
T6 292-408 Sentence denotes Loop-mediated isothermal amplification (LAMP) is an alternative to qPCR that is faster and requires fewer resources.
T7 409-573 Sentence denotes Dao Thi et al. tested the RT-LAMP assay on several hundred clinical RNA samples isolated from pharyngeal swabs collected from individuals being tested for COVID-19.
T8 574-718 Sentence denotes They confirmed that the RT-LAMP assay was a simpler albeit less sensitive option compared to RT-qPCR for large-scale testing for SARS-CoV-2 RNA.
T9 719-962 Sentence denotes These investigators also developed a simplified version of this method (direct swab–to–RT-LAMP assay) that did not require a prior RNA isolation step as well as a method for highly multiplexed sequencing of RT-LAMP reactions (LAMP-sequencing).