| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T149 |
71-113 |
Sentence |
denotes |
(A) Workflow for LAMP-sequencing is shown. |
| T150 |
114-292 |
Sentence |
denotes |
A plate of 96 barcoded (BC) adapters with unique molecular identifiers (UMIs) and mosaic ends (ME) was used as a seed plate for Tn5 tagmentation of all RT-LAMP reaction products. |
| T151 |
293-409 |
Sentence |
denotes |
After tagmentation, each plate was pooled individually, followed by removal of excess adapters using size selection. |
| T152 |
410-755 |
Sentence |
denotes |
Each pool of tagmentation products was then amplified using primers with plate-specific barcodes, and the PCR products were analyzed by Illumina sequencing. (B) Comparison of the outcome of the three assays: LAMP-sequencing (purple, negative; green, positive; gray, too few UMIs), RT-LAMP (after 30-min incubation, y axis), and RT-qPCR (x axis). |
| T153 |
756-787 |
Sentence |
denotes |
Each dot represents one sample. |
| T154 |
788-947 |
Sentence |
denotes |
If a substantial number of the sequencing reads contained SARS-CoV-2 RNA, the sample was called positive (green), if not, then it was called negative (purple). |
| T155 |
948-1250 |
Sentence |
denotes |
For some samples (gray), no LAMP-sequencing call could be made due to too few UMIs. (See also Table 2). (C) Although the RT-LAMP assay was scored after a 30-min incubation at 65°C (left), LAMP-sequencing was performed only after the samples had been incubated for another 10 min (15 min for one plate). |
| T156 |
1251-1432 |
Sentence |
denotes |
This panel shows the RT-LAMP assay outcome (y axis) scored after the full incubation time, whereas the RT-qPCR CT values (x axis) and LAMP-sequencing results are the same as in (B). |
