Id |
Subject |
Object |
Predicate |
Lexical cue |
T101 |
0-294 |
Sentence |
denotes |
In March 2020, at the beginning of the pandemic, the diagnostic laboratory that analyzed the pharyngeal swab samples by RT-qPCR validated all samples that tested positive with the E gene primer set in a second RT-qPCR using the N gene primer set, also of the Sarbeco sets of Corman et al. (15). |
T102 |
295-442 |
Sentence |
denotes |
When plotting RT-LAMP assay results against the CT values for the N gene primer set, we observed a sensitivity cutoff of around CT ≈ 35 (fig. S2A). |
T103 |
443-591 |
Sentence |
denotes |
Direct comparison of the CT values for the E gene and N gene primer sets for all samples revealed a difference of ~5.6 CT units (cycles) (fig. S2B). |
T104 |
592-715 |
Sentence |
denotes |
This suggested that the N gene primers were less sensitive than the E gene primers for detecting SARS-CoV-2 RNA by RT-qPCR. |
T105 |
716-854 |
Sentence |
denotes |
Similar differences have been observed previously for other primer sets, e.g., between the E gene primers and the RdRp-SARSr primers (16). |