| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T83 |
0-100 |
Sentence |
denotes |
Fig. 2 Sensitivity and specificity of the RT-LAMP assay compared to RT-qPCR using clinical samples. |
| T84 |
101-213 |
Sentence |
denotes |
RNA samples isolated from 95 pharyngeal swab specimens were analyzed by the RT-LAMP assay using a 96-well plate. |
| T85 |
214-443 |
Sentence |
denotes |
The RT-LAMP reaction was incubated at 65°C, and the incubation was interrupted at different time points by cooling on ice for 30 s. (A) Photograph of the 96-well plate after a 30-min incubation at 65°C, taken with a mobile phone. |
| T86 |
444-686 |
Sentence |
denotes |
Wells with a yellow color indicate successful RT-LAMP amplification of a fragment of the SARS-CoV-2 N gene (using the N-A primer set). (B) Quantification of the red-to-yellow color change in all wells using spectrophotometric OD measurements. |
| T87 |
687-854 |
Sentence |
denotes |
The color value at the given time points is quantified as the difference between the wavelengths of the two absorbance maxima of phenol red: ΔOD = OD434 nm – OD560 nm. |
| T88 |
855-913 |
Sentence |
denotes |
Yellow (positive) samples yield a ΔOD of about 0.3 to 0.4. |
| T89 |
914-946 |
Sentence |
denotes |
Each line represents one sample. |
| T90 |
947-1183 |
Sentence |
denotes |
For each sample, the line color indicates the CT (cycle threshold) value obtained from RT-qPCR data (using the E-Sarbeco primers) (15). (C) Scatter plot of ΔOD values at the 30-min time point from (B) compared to CT values from RT-qPCR. |
| T91 |
1184-1214 |
Sentence |
denotes |
Each dot is one sample (well). |
