| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T11 |
0-171 |
Sentence |
denotes |
The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) coronavirus is a major public health challenge. |
| T12 |
172-269 |
Sentence |
denotes |
Rapid tests for detecting existing SARS-CoV-2 infections and assessing virus spread are critical. |
| T13 |
270-456 |
Sentence |
denotes |
Approaches to detect viral RNA based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) have potential as simple, scalable, and broadly applicable testing methods. |
| T14 |
457-657 |
Sentence |
denotes |
Compared to RT quantitative polymerase chain reaction (RT-qPCR)–based methods, RT-LAMP assays require incubation at a constant temperature, thus eliminating the need for sophisticated instrumentation. |
| T15 |
658-787 |
Sentence |
denotes |
Here, we tested a two-color RT-LAMP assay protocol for detecting SARS-CoV-2 viral RNA using a primer set specific for the N gene. |
| T16 |
788-936 |
Sentence |
denotes |
We tested our RT-LAMP assay on surplus RNA samples isolated from 768 pharyngeal swab specimens collected from individuals being tested for COVID-19. |
| T17 |
937-1039 |
Sentence |
denotes |
We determined the sensitivity and specificity of the RT-LAMP assay for detecting SARS-CoV-2 viral RNA. |
| T18 |
1040-1276 |
Sentence |
denotes |
Compared to an RT-qPCR assay using a sensitive primer set, we found that the RT-LAMP assay reliably detected SARS-CoV-2 RNA with an RT-qPCR cycle threshold (CT) number of up to 30, with a sensitivity of 97.5% and a specificity of 99.7%. |
| T19 |
1277-1483 |
Sentence |
denotes |
We also developed a swab–to–RT-LAMP assay that did not require a prior RNA isolation step, which retained excellent specificity (99.5%) but showed lower sensitivity (86% for CT < 30) than the RT-LAMP assay. |
| T20 |
1484-1653 |
Sentence |
denotes |
In addition, we developed a multiplexed sequencing protocol (LAMP-sequencing) as a diagnostic validation procedure to detect and record the outcome of RT-LAMP reactions. |
