| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T690 |
0-47 |
Sentence |
denotes |
Crystallization/Soak Protocols for SARS1 with 2 |
| T691 |
48-141 |
Sentence |
denotes |
Crystals of compound 2 bound to SARS1/CoV-1 DC2 protease were produced via cocrystallization. |
| T692 |
142-254 |
Sentence |
denotes |
SARS1/CoV-1 DC2 protease at 10.00 mg/mL was incubated with a 3-fold molar excess of compound 2 for 18 h at 4 °C. |
| T693 |
255-412 |
Sentence |
denotes |
The complex was then passed through a 0.45 μM cellulose acetate spin filter and set up for crystallization using an NT-8 crystallization robot (Formulatrix). |
| T694 |
413-634 |
Sentence |
denotes |
Using MRC-2 crystallization plates, wells containing 40 μL of 4% w/v PEG 6000, 0.1 M MES pH 6.0, and 2.5 mM DTT were dispensed, and then sitting drops consisting of 0.3 μL protein were set up against a 0.3 μL well buffer. |
| T695 |
635-757 |
Sentence |
denotes |
Crystallization plates were incubated at 21 °C, and rectangular crystals measuring 0.1 × 0.25 × 0.25 mm3 grew within 24 h. |
| T696 |
758-892 |
Sentence |
denotes |
Cocrystals were flash-frozen in liquid nitrogen after being passed through a cryo consisting of a well buffer containing 20% glycerol. |
