LitCovid-sentences  

PMC:7571312 / 119052-121008 JSONTXT 6 Projects

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Id Subject Object Predicate Lexical cue
T636 0-43 Sentence denotes SARS CoV-1 Protease FRET Assay and Analysis
T637 44-169 Sentence denotes Proteolytic activity of coronavirus 3CL protease is measured using a continuous fluorescence resonance energy-transfer assay.
T638 170-316 Sentence denotes The SARS 3CLpro FRET assay measures the protease-catalyzed cleavage of TAMRA-SITSAVLQSGFRKMK-(DABCYL)-OH to TAMRA-SITSAVLQ and SGFRKMK(DABCYL)-OH.
T639 317-359 Sentence denotes The fluorescence of the cleaved TAMRA (ex.
T640 360-370 Sentence denotes 558 nm/em.
T641 371-473 Sentence denotes 581 nm) peptide was measured using a TECAN SAFIRE fluorescence plate reader over the course of 10 min.
T642 474-593 Sentence denotes Typical reaction solutions contained 20 mM HEPES (pH 7.0), 1 mM EDTA, 4.0 FRET substrate, 4% DMSO, and 0.005% Tween-20.
T643 594-791 Sentence denotes Assays were initiated with the addition of 25 nM SARS CoV-1 3CLpro nucleotide sequence 9985–10902 of the Urbani strain of SARS coronavirus complete genome sequence (NCBI accession number AY278741).
T644 792-872 Sentence denotes Percent inhibition was determined in duplicate at a 0.001 mM level of inhibitor.
T645 873-1122 Sentence denotes Data was analyzed with the nonlinear regression analysis program KaleidaGraph using the equationwhere offset equals the fluorescence signal of the uncleaved peptide substrate, and limit equals the fluorescence of the fully cleaved peptide substrate.
T646 1123-1263 Sentence denotes The kobs is the first-order rate constant for this reaction and, in the absence of any inhibitor, represents the utilization of a substrate.
T647 1264-1501 Sentence denotes In an enzyme start reaction, which contains an irreversible inhibitor, and where the calculated limit is less than 20% of the theoretical maximum limit, the calculated kobs represents the rate of inactivation of coronavirus 3CL protease.
T648 1502-1605 Sentence denotes The slope (kobs/I) of a plot of kobs vs [I] is a measure of the avidity of the inhibitor for an enzyme.
T649 1606-1730 Sentence denotes For very fast irreversible inhibitors, kobs/I is calculated from observations at only one or two [I] rather than as a slope.
T650 1731-1956 Sentence denotes For the determination of IC50 values for reversible inhibitors, the data was analyzed with the nonlinear regression analysis programs Xlfit3.05 (IDBS, Guildford, U.K.) or GraphPad Prism 8.02 (GraphPad Software San Diego, CA).