| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T94 |
0-302 |
Sentence |
denotes |
To accelerate the clinical diagnostic testing of COVID-19 (particularly for population-based survey or point-of-care testing), a rapid, accurate, and portable detection method based on clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associate system (Cas) has been developed. |
| T95 |
303-435 |
Sentence |
denotes |
The CRISPR/Cas system is an adaptive immune system in archaea and bacteria that defends against foreign genetic elements [47], [48]. |
| T96 |
436-587 |
Sentence |
denotes |
CRISPR allows a programmable protein to attach onto the target site assisted by a guide RNA for cleavage of the target sequence [47], [49], [50], [51]. |
| T97 |
588-856 |
Sentence |
denotes |
CRISPR/Cas12a-based detection has been established together with SARS-CoV-2-specific CRISPR RNAs (crRNAs) targeting the orf1a, orf1b, N, and E genes, and a single-stranded DNA (ssDNA) reporter labeled with a quenched green fluorescent molecule has been developed [48]. |
| T98 |
857-1017 |
Sentence |
denotes |
The fluorescent molecule is cleaved in the presence of a trace amount of SARS-CoV-2 sequences; more importantly, the results can be determined by the naked eye. |
| T99 |
1018-1227 |
Sentence |
denotes |
This system also allows for simultaneous reverse transcription and isothermal amplification at a low temperature, independent of laboratory instruments, and thus could meet the urgent need for rapid diagnosis. |
