T35 |
0-623 |
Sentence |
denotes |
The basic elements of the workflow combine classic cryo-EM algorithms with recent improvements in particle picking (Sanchez-Garcia et al., 2018 ▸; Sanchez-Garcia, Segura et al., 2020 ▸; Wagner et al., 2019 ▸) and the key ideas of meta classifiers, which integrate multiple classifiers by a ‘consensus’ approach (Sorzano et al., 2020 ▸), and finish with a totally new approach to map post-processing based on deep learning that we term Deep cryo-EM Map Enhancer (DeepEMhancer; Sanchez-Garcia, Gomez-Blanco et al., 2020 ▸), which complements our previous proposal on local deblurring (Ramírez-Aportela, Vilas et al., 2020 ▸). |