LitCovid-sentences  

PMC:7546122 / 4113-7573 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T23 0-7 Sentence denotes METHODS
T24 9-26 Sentence denotes IVIG Preparations
T25 27-139 Sentence denotes A total of 54 IVIG lots fractionated from plasma collected prior to the circulation of SARS-CoV-2 were analyzed.
T26 140-444 Sentence denotes The IVIG lots were manufactured from plasma either donated by plasmapheresis (source) or recovered from whole-blood donations (recovered), in the United States (Gammagard Liquid; Baxter Healthcare Corp.; n = 30) or central Europe, that is Austria, Germany, and Czech Republic (KIOVIG; Baxter AG; n = 24).
T27 445-579 Sentence denotes These 54 IVIG lots were tested in 2 independent experiments for nAbs (1 European lot was in a single assay due to volume constraints).
T28 581-601 Sentence denotes Human Plasma Samples
T29 602-691 Sentence denotes Two prepandemic plasma donations were collected in April and May 2019 by BioLife Austria.
T30 692-850 Sentence denotes Human plasma pool samples were generated by the combination of 6 individual donations obtained in the same week at Austrian plasma donation centers (BioLife).
T31 851-1123 Sentence denotes The pools were assembled from donations collected in week 13 (last week of March; n = 40), week 14 (n = 80), week 15 (n = 80), week 16 (n = 80), week 20 (n = 100), week 24 (n = 80), and week 28 (first week of July; n = 100) of 2020, that is during the SARS-CoV-2 pandemic.
T32 1124-1350 Sentence denotes Cumulative incidence of COVID-19 was calculated from data provided by the Austrian Federal Ministry for Social Affairs, Health, Care and Consumer Protection (www.sozialministerium.at) and Statistics Austria (www.statistik.at).
T33 1352-1399 Sentence denotes Detection of SARS-CoV-2 Neutralizing Antibodies
T34 1400-1640 Sentence denotes SARS-CoV-2 nAb titers were determined in IVIG and human plasma samples that were used undiluted, or prediluted with cell culture medium 1:4, 1:5, 1:10, or 1:20 depending on sample amount available, and then serially diluted in 2-fold steps.
T35 1641-1829 Sentence denotes Equal volumes of sample dilutions were mixed with virus stock at 103.0 tissue culture infectious doses 50% per milliliter (TCID50/mL) SARS-CoV-2 (strain BavPat1/2020, kindly provided by C.
T36 1830-1844 Sentence denotes Drosten and V.
T37 1845-1962 Sentence denotes Corman, Charité Berlin, Germany) and incubated for 150 minutes ± 15 minutes, before titration on Vero cells (Cat. no.
T38 1963-2087 Sentence denotes 84113001, European Collection of Authenticated Cell Cultures, Porton Down, Salisbury, UK) in 8-fold replicates per dilution.
T39 2088-2168 Sentence denotes The virus-induced cytopathic effect was determined after 5–7 days of incubation.
T40 2169-2372 Sentence denotes The reciprocal sample dilution resulting in 50% virus neutralization (NT50) was determined using the Spearman-Kärber formula, and the calculated neutralization titer for 50% of the wells reported as 1:X.
T41 2373-2552 Sentence denotes The detection limits were as follows: <1:0.8 for undiluted, <1:3.1 for 1:4 prediluted, <1:3.9 for 1:5 prediluted, <1:7.7 for 1:10 prediluted, and <1:15.4 for 1:20 prediluted IVIG.
T42 2553-2804 Sentence denotes The neutralization assay (µNT) included several validity criteria, that is confirmatory titration of input virus infectivity, cell viability, and neutralization testing of an internal reference standard, all of which had to comply with defined ranges.
T43 2806-2852 Sentence denotes Detection of HCoV-229E Neutralizing Antibodies
T44 2853-3088 Sentence denotes The neutralization assay for HCoV-229E antibodies is essentially identical to the SARS-CoV-2, where samples were used undiluted or prediluted, then serially diluted in 2-fold steps and mixed 1:2 with 103.0 TCID50/mL HCoV-229E (Cat. no.
T45 3089-3202 Sentence denotes VR-740, American Type Culture Collection [ATCC], Rockville, MD), incubated, and titrated on MRC-5 cells (Cat. no.
T46 3203-3233 Sentence denotes CCL-171, ATCC, Rockville, MD).
T47 3234-3314 Sentence denotes The virus-induced cytopathic effect was determined after 7–9 days of incubation.
T48 3316-3347 Sentence denotes Graphs and Statistical Analysis
T49 3348-3460 Sentence denotes Graphical illustration and statistical analysis (paired t tests) were done using GraphPad Prism v8.1.1 software.