PMC:7544934 / 8172-9337 JSON TXT 2 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T66	0-26	Sentence	denotes	ATM isolation and analysis
T67	27-113	Sentence	denotes	Mice were euthanized chemically and epididymal fat was processed for isolation of ATM.
T68	114-226	Sentence	denotes	1 gram of adipose fat was rinsed in PBS and minced to small pieces in HEPES-DMEM buffer containing 10 mg/ml BSA.
T69	227-340	Sentence	denotes	The suspension was centrifuged at 1000 g for 10 min and the resultant supernatant was pipette off to fresh tubes.
T70	341-587	Sentence	denotes	1 mg/ml of collagenase type-IV and 50 U/ml DNAse-II were added to this suspension and incubated at 37 °C for 45 mins with moderate shaking, filtered through 250-micron filter and the resultant solution was centrifuged again at 1000 g for 10 mins.
T71	588-642	Sentence	denotes	Floating cells contained adipocyte and pellet are SVC.
T72	643-756	Sentence	denotes	RBC lysis buffer was added gently to disrupt the sedimented pellets and centrifuged at 1000 g for 10 min at 4 °C.
T73	757-895	Sentence	denotes	Fat macrophage cells were isolated by using BD IMag anti-mouse CD11b + magnetic beads through positive selection under the magnetic field.
T74	896-1031	Sentence	denotes	The percentage purity of macrophage isolation was determined by FACSCANTO II flow cytometer using APC tagged CD11b monoclonal antibody.
T75	1032-1165	Sentence	denotes	The isolated macrophage was processed for RNA isolation, cDNA synthesis and various M1-M2 markers were evaluated using real-time PCR.