Id |
Subject |
Object |
Predicate |
Lexical cue |
T428 |
0-9 |
Sentence |
denotes |
Controls: |
T429 |
10-100 |
Sentence |
denotes |
The imaging workflow was also applied to hydrated and/or healthy lung tissue as a control. |
T430 |
101-165 |
Sentence |
denotes |
First, overview scans covering the entire samples were recorded. |
T431 |
166-348 |
Sentence |
denotes |
Then, hydrated 1 mm biopsy punches (two for CTRLI, one for CTRLII and CTRLIII, where CTRLII and CTRLIII are from the same patient) were recorded in (1 - parallel beam) configuration. |
T432 |
349-431 |
Sentence |
denotes |
Biopsy punches from CTRLII and CTRLIII were also examined in (2 - cone beam) mode. |
T433 |
432-632 |
Sentence |
denotes |
Appendix 3—figure 1 presents (a) the rendering of the hydrated control, and (b,c) virtual slices through the reconstruction volume, showing the lung parenchymal architecture in healthy control tissue. |
T434 |
633-652 |
Sentence |
denotes |
UA-stained samples: |
T435 |
653-757 |
Sentence |
denotes |
For all Covid-19 patients, autopsies were also treated by UA-staining in order to increase the contrast. |
T436 |
758-854 |
Sentence |
denotes |
Stitched overview scans in (1 - parallel beam) configuration were recorded, similar to Figure 3. |
T437 |
855-995 |
Sentence |
denotes |
From the UA-labeled tissue blocks of patients I, III, IV and V, 1 mm, biopsy punches were than scanned in the same configuration (Figure 4). |
T438 |
996-1149 |
Sentence |
denotes |
Using the (2 - cone beam) setup, these samples from I, III and IV were imaged at 8.0keV x-rays, while V was examined at 13.8keV, as shown for Figure 5. |
T439 |
1150-1184 |
Sentence |
denotes |
Variation of propagation distance: |
T440 |
1185-1372 |
Sentence |
denotes |
Scans of the unstained tissue block from patient II were recorded at different propagation distances (z12=50, 100 and 125keV) and different x-ray energies (13.3, 13.8, 14.3 and 14.8 keV). |
T441 |
1373-1474 |
Sentence |
denotes |
In cone-beam configuration, the unstained biopsy punch from patient I was scanned at 13.8keV x-rays. |
T442 |
1475-1493 |
Sentence |
denotes |
Compact μCT scans: |
T443 |
1494-1852 |
Sentence |
denotes |
Prior to the synchrotron experiment, some of the samples have been examined with a laboratory phase-contrast μCT-setup in large mm2-sized FOV-configuration (Liquid metal jet source, Kα=9.5keV, pxeff=5μm, z12=1.7m, 1200 projections of 1 s exposure time with a flat panel CMOS detector with 150μm Gadox-scintillator, PerkinElmer, USA) (Bartels et al., 2013). |
T444 |
1853-2062 |
Sentence |
denotes |
Metal-staining (here UA) of the lung tissue helped to achieve sufficient contrast, similar to previous μCT-studies of other biological tissues (Müller et al., 2017; Busse et al., 2018; De Clercq et al., 2019). |
T445 |
2063-2149 |
Sentence |
denotes |
The resulting overview scans could also be correlated well with histological sections. |