LitCovid-sentences  

PMC:7473770 / 51162-54017 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T426 0-10 Sentence denotes Appendix 3
T427 11-27 Sentence denotes Further datasets
T428 28-37 Sentence denotes Controls:
T429 38-128 Sentence denotes The imaging workflow was also applied to hydrated and/or healthy lung tissue as a control.
T430 129-193 Sentence denotes First, overview scans covering the entire samples were recorded.
T431 194-376 Sentence denotes Then, hydrated 1 mm biopsy punches (two for CTRLI, one for CTRLII and CTRLIII, where CTRLII and CTRLIII are from the same patient) were recorded in (1 - parallel beam) configuration.
T432 377-459 Sentence denotes Biopsy punches from CTRLII and CTRLIII were also examined in (2 - cone beam) mode.
T433 460-660 Sentence denotes Appendix 3—figure 1 presents (a) the rendering of the hydrated control, and (b,c) virtual slices through the reconstruction volume, showing the lung parenchymal architecture in healthy control tissue.
T434 661-680 Sentence denotes UA-stained samples:
T435 681-785 Sentence denotes For all Covid-19 patients, autopsies were also treated by UA-staining in order to increase the contrast.
T436 786-882 Sentence denotes Stitched overview scans in (1 - parallel beam) configuration were recorded, similar to Figure 3.
T437 883-1023 Sentence denotes From the UA-labeled tissue blocks of patients I, III, IV and V, 1 mm, biopsy punches were than scanned in the same configuration (Figure 4).
T438 1024-1177 Sentence denotes Using the (2 - cone beam) setup, these samples from I, III and IV were imaged at 8.0⁢keV x-rays, while V was examined at 13.8⁢keV, as shown for Figure 5.
T439 1178-1212 Sentence denotes Variation of propagation distance:
T440 1213-1400 Sentence denotes Scans of the unstained tissue block from patient II were recorded at different propagation distances (z12=50, 100 and 125keV) and different x-ray energies (13.3, 13.8, 14.3 and 14.8 keV).
T441 1401-1502 Sentence denotes In cone-beam configuration, the unstained biopsy punch from patient I was scanned at 13.8⁢keV x-rays.
T442 1503-1521 Sentence denotes Compact μCT scans:
T443 1522-1880 Sentence denotes Prior to the synchrotron experiment, some of the samples have been examined with a laboratory phase-contrast μCT-setup in large mm2-sized FOV-configuration (Liquid metal jet source, Kα=9.5keV, pxeff=5μm, z12=1.7m, 1200 projections of 1 s exposure time with a flat panel CMOS detector with 150⁢μ⁢m Gadox-scintillator, PerkinElmer, USA) (Bartels et al., 2013).
T444 1881-2090 Sentence denotes Metal-staining (here UA) of the lung tissue helped to achieve sufficient contrast, similar to previous μCT-studies of other biological tissues (Müller et al., 2017; Busse et al., 2018; De Clercq et al., 2019).
T445 2091-2177 Sentence denotes The resulting overview scans could also be correlated well with histological sections.
T446 2178-2198 Sentence denotes Appendix 3—figure 1.
T447 2200-2248 Sentence denotes Illustrations of control lung tissue (hydrated).
T448 2249-2462 Sentence denotes (a) Volume rendering of the tissue block (0.97×1.00×0.73 mm3) and (b) slice through the volume, examined in PB-configuration. (c) Slice from the cone-beam scan, arrows indicating the structure of a healthy septum.
T449 2463-2513 Sentence denotes Particularly, macrophages and erythrocytes emerge.
T450 2514-2554 Sentence denotes Scale bars: (b) 100⁢μ⁢m and (c) 300⁢μ⁢m.
T451 2555-2575 Sentence denotes Appendix 3—figure 2.
T452 2577-2630 Sentence denotes Screening with a laboratory phase-contrast μCT-setup.
T453 2631-2838 Sentence denotes (UA-stained tissue block, patient I). (a) Histological and (b) correlative virtual slice from laboratory phase-contrast tomography. (c) Volume rendering of the entire tissue block from a similar perspective.
T454 2839-2849 Sentence denotes Scale bar:
T455 2850-2855 Sentence denotes 1 mm.