Id |
Subject |
Object |
Predicate |
Lexical cue |
T424 |
0-139 |
Sentence |
denotes |
scATAC-seq targeting 4,000 cells per sample was performed using Chromium Single Cell ATAC Library and Gel Bead kit (10x Genomics, 1000110). |
T425 |
140-208 |
Sentence |
denotes |
Each sample library was uniquely barcoded and quantified by RT-qPCR. |
T426 |
209-442 |
Sentence |
denotes |
Libraries were then pooled and loaded on an Illumina Novaseq 6000 sequencer (3.5 pmol/L loading concentration, 50 + 8 + 16 + 49 bp read configuration) and sequenced to either 90% saturation or 30,000 unique reads per cell on average. |
T427 |
443-674 |
Sentence |
denotes |
All protocols to generate scATAC-seq data on the 10x Chromium platform, including sample preparation, library preparation and instrument and sequencing settings, are available here: https://support.10xgenomics.com/single-cell-atac. |