Id |
Subject |
Object |
Predicate |
Lexical cue |
T337 |
0-63 |
Sentence |
denotes |
Isolation of PBMCs for mass cytometry, scRNA-seq and scATAC-seq |
T338 |
64-295 |
Sentence |
denotes |
For pipeline analysis, venous blood samples were derived from each healthy donor or patient using Ficoll-Hypaque density solution, heparinized and then processed by standard density gradient centrifugation methods to isolate PBMCs. |
T339 |
296-393 |
Sentence |
denotes |
The viability and quantity of PBMCs in single-cell suspensions were determined using Trypan Blue. |
T340 |
394-443 |
Sentence |
denotes |
For each sample, the cell viability exceeded 90%. |
T341 |
444-623 |
Sentence |
denotes |
For each sample with more than 1 × 107 viable cells, a fraction of PBMCs was extracted for scRNA-seq analysis, a fraction of PBMCs was allocated for scATAC-seq and mass cytometry. |