PMC:7417788 / 61393-62016 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T337 0-63 Sentence denotes Isolation of PBMCs for mass cytometry, scRNA-seq and scATAC-seq
T338 64-295 Sentence denotes For pipeline analysis, venous blood samples were derived from each healthy donor or patient using Ficoll-Hypaque density solution, heparinized and then processed by standard density gradient centrifugation methods to isolate PBMCs.
T339 296-393 Sentence denotes The viability and quantity of PBMCs in single-cell suspensions were determined using Trypan Blue.
T340 394-443 Sentence denotes For each sample, the cell viability exceeded 90%.
T341 444-623 Sentence denotes For each sample with more than 1 × 107 viable cells, a fraction of PBMCs was extracted for scRNA-seq analysis, a fraction of PBMCs was allocated for scATAC-seq and mass cytometry.