LitCovid-sentences  

PMC:7417788 / 51645-53782 JSONTXT 11 Projects

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Id Subject Object Predicate Lexical cue
T261 0-142 Sentence denotes Numerous studies have reported important observations about the composition and functional alterations of immune cells in animal aging models.
T262 143-227 Sentence denotes However, animal models fail to recapitulate the human immune environment adequately.
T263 228-382 Sentence denotes What we know about human immune cells is primarily based on flow cytometric analysis, relying on previously described markers for pooled cell populations.
T264 383-495 Sentence denotes These analytical methods are too biased to reveal information on selected and not all cells or cell populations.
T265 496-732 Sentence denotes Single-cell technologies open new avenues in many research fields but are particularly important for analyzing human cells in aging and diseases in an unbiased and global fashion (He et al., 2020; Wang et al., 2020; Zhang et al., 2020).
T266 733-1110 Sentence denotes Using scRNA-seq, recent studies have reported transcriptomic and functional changes in immune cells during aging in mouse cells and tissues such as the central nervous system (Mrdjen et al., 2018), macrophages in brain (Martinez-Jimenez et al., 2017; Van Hove et al., 2019), TCs in spleen (Dulken et al., 2019), and hematopoietic stem cells in bone marrow (Leins et al., 2018).
T267 1111-1202 Sentence denotes Recently, our group revealed how aging affects the immune system in rats (Ma et al., 2020).
T268 1203-1339 Sentence denotes For humans, mass cytometry analysis showed that aging increased epigenetic variations in circulatory immune cells (Cheung et al., 2018).
T269 1340-1421 Sentence denotes However, a comprehensive atlas of immune cell aging has not yet been constructed.
T270 1422-1561 Sentence denotes Here, we depicted such an atlas from PBMCs harvested from healthy young and old research subjects and young and old patients with COVID-19.
T271 1562-1677 Sentence denotes First, scRNA-seq and CyTOF reveal that aging causes cell compositional changes at the cell type and subtype levels.
T272 1678-1817 Sentence denotes Second, our study provides the first high-quality analysis of TCR and BCR repertoires in young and aged adults at a single-cell resolution.
T273 1818-1980 Sentence denotes Third, our study provides the first chromosomal accessibility profiles of major immune cells in young and aged healthy research subjects at the single-cell level.
T274 1981-2137 Sentence denotes Combined with several novel single-cell methodologies, this study represents a state-of-the-art unbiased and systematic analysis of human immune cell aging.