| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T415 |
0-46 |
Sentence |
denotes |
Intracellular stain after CD3/CD28 stimulation |
| T416 |
47-160 |
Sentence |
denotes |
96-well flat bottom plates were coated with 1μg/mL of anti-CD3 (UCHT1, #BE0231, BioXell) in PBS at 4°C overnight. |
| T417 |
161-271 |
Sentence |
denotes |
The next day, cells were collected and plated at 1 × 105/well in 100 μl with 1/1000 of GolgiPlug (BD #555029). |
| T418 |
272-384 |
Sentence |
denotes |
2μg/mL of anti-human CD28/CD49d was added to the wells containing plate-bound anti-CD3 (Clone L293, 347690, BD). |
| T419 |
385-529 |
Sentence |
denotes |
GolgiPlug-treated PBMCs were stimulated or left unstimulated for 16 hours, spun down (1200 rpm, 10 min) and were stained for intracellular IFNɣ. |
