LitCovid-sentences  

PMC:7402624 / 64080-65577 JSONTXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T379 0-28 Sentence denotes Antibody panels and staining
T380 29-106 Sentence denotes Approximately 1-5×106 freshly isolated PBMCs were used per patient per stain.
T381 107-187 Sentence denotes See table S7 for buffer information and table S8 for antibody panel information.
T382 188-309 Sentence denotes PBMCs were stained with live/dead mix (100 μl, 10 min, RT), washed with FACS buffer, and spun down (1500 rpm, 5 min, RT).
T383 310-424 Sentence denotes PBMCs were incubated with 100 μl of Fc block (RT, 10 min) before a second wash (FACS buffer, 1500 rpm, 5 min, RT).
T384 425-526 Sentence denotes Pellet was resuspended in 25 μl of chemokine receptor staining mix, and incubated at 37°C for 20 min.
T385 527-663 Sentence denotes Following incubation, 25 μl of surface receptor staining mix was directly added and the PBMCs were incubated at RT for a further 45 min.
T386 664-837 Sentence denotes PBMCs were washed (FACS buffer, 1500 rpm, 5 min, RT) and stained with 50 μl of secondary antibody mix for 20 min at RT, then washed again (FACS buffer, 1500 rpm, 5 min, RT).
T387 838-979 Sentence denotes Samples were fixed and permeabilized by incubating in 100 μl of Fix/Perm buffer (RT, 30 min) and washed in Perm Buffer (1800 rpm, 5 min, RT).
T388 980-1047 Sentence denotes PBMCs were stained with 50μl of intracellular mix overnight at 4°C.
T389 1048-1163 Sentence denotes The following morning, samples were washed (Perm Buffer, 1800 rpm, 5 min, RT) and further fixed in 50 μl of 4% PFA.
T390 1164-1277 Sentence denotes Prior to acquisition, samples were diluted to 1% PFA and 10,000 counting beads added per sample (BD, Cat#335925).
T391 1278-1312 Sentence denotes Live/dead mix was prepared in PBS.
T392 1313-1451 Sentence denotes For the surface receptor and chemokine staining mix, antibodies were diluted in FACS buffer with 50% BD Brilliant Buffer (BD, Cat#566349).
T393 1452-1497 Sentence denotes Intracellular mix was diluted in Perm Buffer.