| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T65 |
0-284 |
Sentence |
denotes |
For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. |
| T66 |
285-360 |
Sentence |
denotes |
Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. |
| T67 |
361-555 |
Sentence |
denotes |
Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). |
| T68 |
556-755 |
Sentence |
denotes |
Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). |
| T69 |
756-934 |
Sentence |
denotes |
Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. |
| T70 |
935-1008 |
Sentence |
denotes |
Each channel was collected separately, with images at 1024 × 1024 pixels. |
