| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T46 |
0-39 |
Sentence |
denotes |
Purification of monoclonal antibody 1A9 |
| T47 |
40-96 |
Sentence |
denotes |
The hybridoma for mAb 1A9 was previously generated [20]. |
| T48 |
97-259 |
Sentence |
denotes |
All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. |
| T49 |
260-378 |
Sentence |
denotes |
The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. |
| T50 |
379-506 |
Sentence |
denotes |
The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific). |
