LitCovid-sentences  

PMC:7376845 / 27110-30398 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T213 0-10 Sentence denotes Discussion
T214 11-127 Sentence denotes Numerous mAbs against the S protein of SARS-CoV-1 have been generated for research and diagnostic assay development.
T215 128-265 Sentence denotes Some of these may be able to cross-react with the S protein of SARS-CoV-2 and serve as tools to aid research on this newly emerged virus.
T216 266-431 Sentence denotes In this current study, an immunogenic domain in the S2 subunit of SARS-CoV-1 was found to be highly conserved in multiple strains of SARS-CoV-2 (Figure 1 and Table).
T217 432-610 Sentence denotes Consistently, WB and IF analyses showed that four different mAbs generated using this SARS-CoV-1 domain were cross-reactive against the S protein of SARS-CoV-2 (Figures 2 and 3).
T218 611-736 Sentence denotes Recent cross-reactivity studies have evaluated SARS-CoV-1 neutralising antibodies that bind to the RBD-containing S1 subunit.
T219 737-909 Sentence denotes Although both SARS-CoV-1 and SARS-CoV-2 use ACE2 as a receptor for viral entry [3,16], several SARS-CoV-1 RBD-directed mAbs did not cross-react with SARS-CoV-2 RBD [28,29].
T220 910-1113 Sentence denotes Interestingly, CR3022, which was isolated from a SARS convalescent patient [22], showed cross-reactivity to SARS-CoV-2 RBD and recognises an epitope that does not overlap with the ACE2 binding site [28].
T221 1114-1246 Sentence denotes Among the four mAbs tested in this study, indirect ELISA showed that 1A9 binds strongest to the S protein of SARS-CoV-2 (Figure 3B).
T222 1247-1414 Sentence denotes To determine if 1A9 is useful for detection of S protein in a sandwich ELISA, it was paired with CR3022 since 1A9 binds to S2 subunit while CR3022 binds to S1 subunit.
T223 1415-1520 Sentence denotes As would be expected, these two antibodies can be paired to detect S protein from 15.6 ng/mL (Figure 4A).
T224 1521-1731 Sentence denotes In addition, mAb 1A9 stained a considerable number of SARS-CoV-2-infected cells at 24 hours post-infection showing that it is sensitive enough to detect the expression of S protein during infection (Figure 4B).
T225 1732-1873 Sentence denotes Thus, mAbs 1A9 will be useful for studying the kinetics of SARS-CoV-2 replication in vitro and development of diagnostic assays for COVID-19.
T226 1874-2021 Sentence denotes It is noteworthy that cytotoxic T-lymphocyte (CTL) epitopes also reside at residues 884–891 and 1116–1123 within the S2 subunit of SARS-CoV-1 [30].
T227 2022-2113 Sentence denotes Interestingly, the latter CTL epitope overlaps with the epitope recognised by mAb 1A9 [21].
T228 2114-2264 Sentence denotes Hence, the S2 subunit may serve as an important antigen for inducing both humoral as well as cell-mediated immunity against SARS-CoV-1 and SARS-CoV-2.
T229 2265-2484 Sentence denotes To our knowledge, this is the first study showing that mAbs targeting the S2 domain of SARS-CoV-1 can cross-react with SARS-CoV-2 and this observation is consistent with the high sequence conservation in the S2 subunit.
T230 2485-2691 Sentence denotes The ability of these antibodies, particularly 1A9, to detect SARS-CoV-2 S protein in indirect and sandwich ELISAs demonstrate their utility for detection of SARS-CoV-2 infections in a public health setting.
T231 2692-2912 Sentence denotes Whether or not the current sensitivity of these antibodies are sufficient for robust detection of SARS-CoV-2 infections in a clinical setting and how they compare to existing PCR-based detection remains to be determined.
T232 2913-3060 Sentence denotes Successful development of these antibodies into a point of care diagnostic kit will provide a complementary approach to existing detection methods.
T233 3061-3196 Sentence denotes Besides the mAbs characterised here, several other mAbs have been reported to bind to epitopes in the S2 subunit of SARS-CoV-1 [31-33].
T234 3197-3288 Sentence denotes Thus, it will be important to determine if these mAbs can also cross-react with SARS-CoV-2.