LitCovid-sentences  

PMC:7376845 / 22269-24219 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T170 0-228 Sentence denotes Figure 3 Antibodies expected to target SARS-CoV-2 S protein, (A) hybridise to the denatured protein in western blot, (B) bind to the protein in ELISA and (C) recognise cells expressing the protein as shown by immunofluorescence
T171 229-234 Sentence denotes DAPI:
T172 235-465 Sentence denotes 4′,6-diamidino-2-phenylindole; mAb: monoclonal antibody: OD: optical density; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; S protein: spike protein; vec: pXJ40-Myc expression vector used as an empty vector control.
T173 466-468 Sentence denotes A.
T174 469-575 Sentence denotes Each photo depicts a western blot using the primary antibody indicated below it (2B2, 1A9, 4B12, or 1G10).
T175 576-772 Sentence denotes Empty vector-transfected 293FT cell products are on the lanes labelled as ‘Vec’, while products of 293FT cells expressing full-length SARS-CoV-2 S protein are on the lanes labelled ‘SARS-CoV-2 S’.
T176 773-866 Sentence denotes Primary antibodies were labelled with horseradish peroxidase-conjugated secondary antibodies.
T177 867-1022 Sentence denotes A ladder indicative of the molecular weights in kD of the proteins relative to their vertical position on the blots, is indicated on the left of the panel.
T178 1023-1025 Sentence denotes B.
T179 1026-1145 Sentence denotes The abilities of 2B2, 1A9, 4B12 and 1G10 monoclonal antibodies to bind to SARS-CoV-2 S protein was determined by ELISA.
T180 1146-1264 Sentence denotes Individual wells were coated with 20 ng of SARS-CoV-2 S protein and incubated with serially diluted mAbs as indicated.
T181 1265-1446 Sentence denotes A representative plot from three independent experiment is show for each antibody and error bars correspond to standard deviations of each mAb experiment carried out in triplicates.
T182 1447-1449 Sentence denotes C.
T183 1450-1605 Sentence denotes Each photo depicts an immunofluorescence assay using either no primary antibody, or the primary antibody indicated below it (Myc, 2B2, 1A9, 4B12, or 1G10).
T184 1606-1771 Sentence denotes Immunofluorescence analysis was performed on empty vector-transfected COS-7 cells (top photos) and cells expressing full-length SARS-CoV-2 S protein (bottom photos).
T185 1772-1881 Sentence denotes The indicated primary antibodies were used followed by Alexa Fluor 488-conjugated secondary antibody (green).
T186 1882-1931 Sentence denotes Cell nuclei were counterstained with DAPI (blue).
T187 1932-1950 Sentence denotes Scale bar = 50 µm.