PMC:7376845 / 20322-21937 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T154 0-218 Sentence denotes Figure 2 Monoclonal antibodies expected to target a SARS-CoV-2 S protein S2 fragment, (A) hybridise to the peptide fragment in western blot and (B) recognise cells expressing the peptide as shown by immunofluorescence
T155 219-279 Sentence denotes aa: amino acid; HBcAg: hepatitis B virus core antigen; DAPI:
T156 280-463 Sentence denotes 4′,6-diamidino-2-phenylindole; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; S protein: spike protein; vec: pXJ40-Myc expression vector used as an empty vector control.
T157 464-466 Sentence denotes A.
T158 467-578 Sentence denotes Each photo depicts a western blot using the primary antibody indicated below it (Myc, 2B2, 1A9, 4B12, or 1G10).
T159 579-853 Sentence denotes Empty vector-transfected 293FT cell products are on the lanes labelled as ‘Vec’, while products of 293FT cells expressing Myc-tagged SARS-CoV-2 S protein fragment (aa 1048–1206; SARS-CoV-2 numbering) or Myc-tagged HBcAg are on the lanes respectively labelled ‘S’ or ‘HBcAg’.
T160 854-947 Sentence denotes Primary antibodies were labelled with horseradish peroxidase-conjugated secondary antibodies.
T161 948-1103 Sentence denotes A ladder indicative of the molecular weights in kD of the proteins relative to their vertical position on the blots, is indicated on the left of the panel.
T162 1104-1106 Sentence denotes B.
T163 1107-1262 Sentence denotes Each photo depicts an immunofluorescence assay using either no primary antibody, or the primary antibody indicated below it (Myc, 2B2, 1A9, 4B12, or 1G10).
T164 1263-1546 Sentence denotes Immunofluorescence assay results of COS7 cells expressing Myc-tagged HBcAg (top photos) or a Myc-tagged fragment (aa1048–1206) of SARS-CoV-2 S protein (bottom photos) using the indicated primary antibodies, followed by Alexa Fluor 488-conjugated secondary antibody (green) are shown.
T165 1547-1596 Sentence denotes Cell nuclei were counterstained with DAPI (blue).
T166 1597-1615 Sentence denotes Scale bar = 50 µm.