Id |
Subject |
Object |
Predicate |
Lexical cue |
T154 |
0-218 |
Sentence |
denotes |
Figure 2 Monoclonal antibodies expected to target a SARS-CoV-2 S protein S2 fragment, (A) hybridise to the peptide fragment in western blot and (B) recognise cells expressing the peptide as shown by immunofluorescence |
T155 |
219-279 |
Sentence |
denotes |
aa: amino acid; HBcAg: hepatitis B virus core antigen; DAPI: |
T156 |
280-463 |
Sentence |
denotes |
4′,6-diamidino-2-phenylindole; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; S protein: spike protein; vec: pXJ40-Myc expression vector used as an empty vector control. |
T157 |
464-466 |
Sentence |
denotes |
A. |
T158 |
467-578 |
Sentence |
denotes |
Each photo depicts a western blot using the primary antibody indicated below it (Myc, 2B2, 1A9, 4B12, or 1G10). |
T159 |
579-853 |
Sentence |
denotes |
Empty vector-transfected 293FT cell products are on the lanes labelled as ‘Vec’, while products of 293FT cells expressing Myc-tagged SARS-CoV-2 S protein fragment (aa 1048–1206; SARS-CoV-2 numbering) or Myc-tagged HBcAg are on the lanes respectively labelled ‘S’ or ‘HBcAg’. |
T160 |
854-947 |
Sentence |
denotes |
Primary antibodies were labelled with horseradish peroxidase-conjugated secondary antibodies. |
T161 |
948-1103 |
Sentence |
denotes |
A ladder indicative of the molecular weights in kD of the proteins relative to their vertical position on the blots, is indicated on the left of the panel. |
T162 |
1104-1106 |
Sentence |
denotes |
B. |
T163 |
1107-1262 |
Sentence |
denotes |
Each photo depicts an immunofluorescence assay using either no primary antibody, or the primary antibody indicated below it (Myc, 2B2, 1A9, 4B12, or 1G10). |
T164 |
1263-1546 |
Sentence |
denotes |
Immunofluorescence assay results of COS7 cells expressing Myc-tagged HBcAg (top photos) or a Myc-tagged fragment (aa1048–1206) of SARS-CoV-2 S protein (bottom photos) using the indicated primary antibodies, followed by Alexa Fluor 488-conjugated secondary antibody (green) are shown. |
T165 |
1547-1596 |
Sentence |
denotes |
Cell nuclei were counterstained with DAPI (blue). |
T166 |
1597-1615 |
Sentence |
denotes |
Scale bar = 50 µm. |