LitCovid-sentences  

PMC:7376845 / 1819-5686 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T19 0-12 Sentence denotes Introduction
T20 13-222 Sentence denotes The severe acute respiratory syndrome coronavirus (SARS-CoV-1), a virus considered to have a zoonotic origin, is the aetiological agent for the infectious disease, SARS, which first emerged in 2002–2003 [1,2].
T21 223-488 Sentence denotes In December of 2019, another novel coronavirus (SARS-CoV-2), which causes coronavirus disease (COVID-19), appeared to have crossed species barriers to infect humans and was effectively transmitted from person to person, leading to an outbreak in Wuhan, China [3-5].
T22 489-618 Sentence denotes This virus subsequently spread worldwide, leading the World Health Organization (WHO) to declare a pandemic on 11 March 2020 [6].
T23 619-795 Sentence denotes To date, SARS-CoV-2 continues to pose a high global health and economy burden, and as at 3 May 2020, COVID-19 had affected 215 countries with over 3.35 million confirmed cases.
T24 796-919 Sentence denotes To tackle the problems caused by SARS-CoV-2, improving its detection and knowledge of its infection mechanism is important.
T25 920-1062 Sentence denotes In this respect, the viral surface spike glycoprotein (S protein) has been demonstrated to play key role in host cell selectivity and binding.
T26 1063-1319 Sentence denotes The S protein is functionally divided into two subunits, with the S1 subunit containing the receptor binding domain (RBD), which allows attachment to host cells, and the S2 subunit mediating fusion between viral and host membranes (reviewed by Li, F.) [7].
T27 1320-1490 Sentence denotes Phylogenetic analysis revealed that like SARS-CoV-1 and bat-derived SARS-like coronaviruses (SL-CoVs), SARS-CoV-2 belongs to lineage B of the betacoronavirus genus [8,9].
T28 1491-1711 Sentence denotes A study of 56 complete and partial SARS-CoV-2 genomes isolated from COVID-19 patients showed very high sequence conservation of more than 99%, indicating a recent introduction of the virus into the human population [10].
T29 1712-1847 Sentence denotes Although the animal source of SARS-CoV-2 is not clear, SARS-CoV-1 is believed to have originated from SL-CoVs residing in bats [11-14].
T30 1848-1999 Sentence denotes For the majority of SL-CoVs, the S1 subunit has low sequence identity to that of SARS-CoV-1, which suggests species-dependent receptor binding [14,15].
T31 2000-2176 Sentence denotes On the other hand, the high amino acid sequence identity of more than 90% in the S2 subunit suggests that the fusion mechanism during virus infection is well-conserved [14,15].
T32 2177-2381 Sentence denotes While SARS-CoV-2 shares higher whole-genome sequence identity with bat-SL-CoVZC45 and bat-SL-CoVZXC21 (88–89%) than with SARS-CoV-1 (79–82%), the RBD of SARS-CoV-2 is more similar to SARS-CoV-1 RBD [8,9].
T33 2382-2572 Sentence denotes In line with this, several research groups have demonstrated that SARS-CoV-2 utilises the same host receptor, angiotensin-converting enzyme 2 (ACE2), as SARS-CoV-1 for viral entry [3,16-18].
T34 2573-2689 Sentence denotes Due to its role in virus entry, the S protein has been the target for the generation of monoclonal antibodies (mAb).
T35 2690-2789 Sentence denotes In our previous work, we used five different fragments of SARS-CoV-1 S protein to immunise rabbits.
T36 2790-2943 Sentence denotes A fragment corresponding to residues 1029 to 1192 in the S2 subunit of SARS-CoV-1 was found to stimulate neutralising antibodies against SARS-CoV-1 [19].
T37 2944-3100 Sentence denotes This fragment was subsequently used to generate a panel of murine mAbs with their respective binding domains characterised and described in Lip et al. [20].
T38 3101-3392 Sentence denotes One of them, mAb 1A9, which binds to the S protein through a recently identified epitope within the S2 subunit at amino acids 1111–1130, has the ability to bind and cross-neutralise pseudotyped viruses expressing the S protein of human SARS-CoV-1, civet SARS-CoV and bat SL-CoV strains [21].
T39 3393-3641 Sentence denotes In this study, we aim to verify if the sequence of the immunogen used to generate mAb 1A9, as well as three other mAbs, is conserved in different coronaviruses and if these mAbs bind to the S protein of SARS-CoV-2 expressed in mammalian cell lines.
T40 3642-3867 Sentence denotes Importantly, mAb 1A9 is investigated for its ability to detect the S protein in SARS-CoV-2 infected cells and purified S protein in a sandwich ELISA format when paired with another mAb binding to the S1 subunit of SARS-CoV-2.