LitCovid-sentences  

PMC:7376845 / 120-1817 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3 0-10 Sentence denotes Background
T4 11-141 Sentence denotes A novel coronavirus, SARS-CoV-2, which emerged at the end of 2019 and causes COVID-19, has resulted in worldwide human infections.
T5 142-392 Sentence denotes While genetically distinct, SARS-CoV-1, the aetiological agent responsible for an outbreak of severe acute respiratory syndrome (SARS) in 2002–2003, utilises the same host cell receptor as SARS-CoV-2 for entry: angiotensin-converting enzyme 2 (ACE2).
T6 393-507 Sentence denotes Parts of the SARS-CoV-1 spike glycoprotein (S protein), which interacts with ACE2, appear conserved in SARS-CoV-2.
T7 509-512 Sentence denotes Aim
T8 513-652 Sentence denotes The cross-reactivity with SARS-CoV-2 of monoclonal antibodies (mAbs) previously generated against the S protein of SARS-CoV-1 was assessed.
T9 654-661 Sentence denotes Methods
T10 662-802 Sentence denotes The SARS-CoV-2 S protein sequence was aligned to those of SARS-CoV-1, Middle East respiratory syndrome (MERS) and common-cold coronaviruses.
T11 803-1030 Sentence denotes Abilities of mAbs generated against SARS-CoV-1 S protein to bind SARS-CoV-2 or its S protein were tested with SARS-CoV-2 infected cells as well as cells expressing either the full length protein or a fragment of its S2 subunit.
T12 1031-1121 Sentence denotes Quantitative ELISA was also performed to compare binding of mAbs to recombinant S protein.
T13 1123-1130 Sentence denotes Results
T14 1131-1281 Sentence denotes An immunogenic domain in the S2 subunit of SARS-CoV-1 S protein is highly conserved in SARS-CoV-2 but not in MERS and human common-cold coronaviruses.
T15 1282-1411 Sentence denotes Four murine mAbs raised against this immunogenic fragment could recognise SARS-CoV-2 S protein expressed in mammalian cell lines.
T16 1412-1552 Sentence denotes In particular, mAb 1A9 was demonstrated to detect S protein in SARS-CoV-2-infected cells and is suitable for use in a sandwich ELISA format.
T17 1554-1564 Sentence denotes Conclusion
T18 1565-1697 Sentence denotes The cross-reactive mAbs may serve as useful tools for SARS-CoV-2 research and for the development of diagnostic assays for COVID-19.