PMC:7376845 / 11841-13021 JSON TXT 6 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id	Subject	Object	Predicate	Lexical cue
T83	0-182	Sentence	denotes	Mab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C.
T84	183-289	Sentence	denotes	The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min.
T85	290-387	Sentence	denotes	Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number:
T86	388-464	Sentence	denotes	40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number:
T87	465-574	Sentence	denotes	11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST.
T88	575-804	Sentence	denotes	100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C.
T89	805-930	Sentence	denotes	Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid.
T90	931-991	Sentence	denotes	The OD450nm was determined by a Tecan Infinite M1000 reader.
T91	992-1180	Sentence	denotes	Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values < 0.05 were considered statistically significant.