Id |
Subject |
Object |
Predicate |
Lexical cue |
T78 |
0-40 |
Sentence |
denotes |
Production of monoclonal antibody CR3022 |
T79 |
41-125 |
Sentence |
denotes |
The human mAb CR3022 was expressed in a similar manner as previously described [22]. |
T80 |
126-447 |
Sentence |
denotes |
The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. |
T81 |
448-634 |
Sentence |
denotes |
Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]. |