PMC:7376845 / 11190-11824 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T78 0-40 Sentence denotes Production of monoclonal antibody CR3022
T79 41-125 Sentence denotes The human mAb CR3022 was expressed in a similar manner as previously described [22].
T80 126-447 Sentence denotes The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively.
T81 448-634 Sentence denotes Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23].