| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T59 |
0-37 |
Sentence |
denotes |
Sanger sequencing of bat coronavirus: |
| T60 |
38-132 |
Sentence |
denotes |
The RT-PCR products were separated on 1.5 per cent agarose gel, and 440 bp bands were excised. |
| T61 |
133-240 |
Sentence |
denotes |
The excised gels were extracted and purified using a QIAQuick gel extraction kit (Qiagen, Hilden, Germany). |
| T62 |
241-456 |
Sentence |
denotes |
The purified products were quantified, and chain-terminated PCR reactions were performed using pathogen-specific forward and reverse primers21 with the BigDye Terminator 3.1 sequencing kit (Applied Biosystems, USA). |
| T63 |
457-535 |
Sentence |
denotes |
BigDye reactions were purified using the DyeEx 2.0 spin kit (Qiagen, Germany). |
| T64 |
536-673 |
Sentence |
denotes |
The purified chain-terminated reactions were sequenced using the ABI PRISM® 3100 Automated DNA Sequencer (Thermo Fisher Scientific, USA). |
| T65 |
674-771 |
Sentence |
denotes |
The sequence data generated were assembled using the Sequencer 5.1 software (Accelrys Inc., USA). |
