| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T55 |
0-150 |
Sentence |
denotes |
Detection of bat coronavirus using RT-PCR: RNA was extracted from the bat specimens using the MagMAX pathogen RNA/DNA isolation kit (Invitrogen, USA). |
| T56 |
151-409 |
Sentence |
denotes |
RT-PCR was performed using Superscript III one-step RT-PCR (Invitrogen, USA) with Platinum High-Fidelity Taq polymerase (Invitrogen, USA) using the published BtCoV-specific primers targeting the conserved region of RNA-dependent RNA polymerase (RdRp) gene21. |
| T57 |
410-557 |
Sentence |
denotes |
The amplicon of 440 bp was separated on 1.5 per cent agarose gel and visualized under VersaDoc MP 4000 ultraviolet transilluminator (Bio-Rad, USA). |
