LitCovid-sentences  

PMC:7366549 / 108-2735 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T3 0-24 Sentence denotes Background & objectives:
T4 25-135 Sentence denotes Bats are considered to be the natural reservoir for many viruses, of which some are potential human pathogens.
T5 136-231 Sentence denotes In India, an association of Pteropus medius bats with the Nipah virus was reported in the past.
T6 232-370 Sentence denotes It is suspected that the recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) also has its association with bats.
T7 371-582 Sentence denotes To assess the presence of CoVs in bats, we performed identification and characterization of bat CoV (BtCoV) in P. medius and Rousettus species from representative States in India, collected during 2018 and 2019.
T8 584-592 Sentence denotes Methods:
T9 593-838 Sentence denotes Representative rectal swab (RS) and throat swab specimens of Pteropus and Rousettus spp. bats were screened for CoVs using a pan-CoV reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene.
T10 839-918 Sentence denotes A single-step RT-PCR was performed on the RNA extracted from the bat specimens.
T11 919-1030 Sentence denotes Next-generation sequencing (NGS) was performed on a few representative bat specimens that were tested positive.
T12 1031-1209 Sentence denotes Phylogenetic analysis was carried out on the partial sequences of RdRp gene sequences retrieved from both the bat species and complete viral genomes recovered from Rousettus spp.
T13 1211-1219 Sentence denotes Results:
T14 1220-1373 Sentence denotes Bat samples from the seven States were screened, and the RS specimens of eight Rousettus spp. and 21 Pteropus spp. were found positive for CoV RdRp gene.
T15 1374-1505 Sentence denotes Among these, by Sanger sequencing, partial RdRp sequences could be retrieved from three Rousettus and eight Pteropus bat specimens.
T16 1506-1668 Sentence denotes Phylogenetic analysis of the partial RdRp region demonstrated distinct subclustering of the BtCoV sequences retrieved from these Rousettus and Pteropus spp. bats.
T17 1669-1802 Sentence denotes NGS led to the recovery of four sequences covering approximately 94.3 per cent of the whole genome of the BtCoVs from Rousettus bats.
T18 1803-1880 Sentence denotes Three BtCoV sequences had 93.69 per cent identity to CoV BtRt-BetaCoV/GX2018.
T19 1881-1951 Sentence denotes The fourth BtCoV sequence was 96.8 per cent identical to BtCoV HKU9-1.
T20 1953-1982 Sentence denotes Interpretation & conclusions:
T21 1983-2062 Sentence denotes This study was a step towards understanding the CoV circulation in Indian bats.
T22 2063-2186 Sentence denotes Detection of potentially pathogenic CoVs in Indian bats stresses the need for enhanced screening for novel viruses in them.
T23 2187-2349 Sentence denotes One Health approach with collaborative activities by the animal health and human health sectors in these surveillance activities shall be of use to public health.
T24 2350-2487 Sentence denotes This would help in the development of diagnostic assays for novel viruses with outbreak potential and be useful in disease interventions.
T25 2488-2627 Sentence denotes Proactive surveillance remains crucial for identifying the emerging novel viruses with epidemic potential and measures for risk mitigation.