LitCovid-sentences  

PMC:7358770 / 10646-14450 JSONTXT 13 Projects

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Id Subject Object Predicate Lexical cue
T67 0-38 Sentence denotes 2.2 Anti-coronavirus activity of GAGs
T68 39-201 Sentence denotes Cell surface GAGs serve as co-receptors by increasing the local concentration of pathogens, so that they can more efficiently interact with their entry receptors.
T69 202-340 Sentence denotes Most coronavirus receptors of carbohydrate are mainly negatively charged, such as sulfated GAGs or glycans containing sialic acid [54,55].
T70 341-525 Sentence denotes S protein concentrated outside the virus contains the receptor binding domains (RBDs) at the N-terminal, such as MHV-CoV N-RBD and SARS C-RBD with their receptor (Fig. 2B–D) [7,56,57].
T71 526-687 Sentence denotes The coronavirus NL63 (CoV-NL63), and SARS-CoV use angiotensin-converting enzyme 2 (ACE2) as a primary receptor for infection of target cells (Fig. 2) [56,58,59].
T72 688-859 Sentence denotes Phylogenetically, SARS-CoV-2 is almost identical to SARS-CoV, sharing 79.6% genomic sequence identity [60], and use the same cell entry receptor, ACE2, as SARS-CoV [8,60].
T73 860-1082 Sentence denotes During infection, CoV first binds host cell via interaction between its S1-RBD and the cell membrane receptor, triggering conformational changes in the S2 subunit that result in virus fusion and entry into the target cell.
T74 1083-1242 Sentence denotes Viral RNA gradually forms mature virions through replication, transcription, and synthesis, and then is released from host cell (Fig. 4 ) [8,[60], [61], [62]].
T75 1243-1406 Sentence denotes However, the expression of ACE2 is not sufficient for infection, and HSPGs play important roles in the entry of some pathogens such as SARS-CoV [[63], [64], [65]].
T76 1407-1508 Sentence denotes A soluble HS was used to assess whether the attachment of HCoV-NL63 was mediated by HS proteoglycans.
T77 1509-1748 Sentence denotes Flow cytometric analysis showed that the adhesion of virus to LLC-Mk2 cells was completely inhibited in the presence of soluble HS, indicating the role of this molecule in adhesion to susceptible cells and possible also in cell entry [54].
T78 1749-1935 Sentence denotes Both CoV-NL63 and SARS-CoV initially bind to the HS on the cell surface, and virus entry depends on the HS interaction, indicating that HS can inhibit virus attachment and entry [22,54].
T79 1936-1986 Sentence denotes Fig. 4 Life cycle of highly pathogenic human CoVs.
T80 1987-2097 Sentence denotes These CoVs enter host cells by first binding to their respective cellular receptors via the surface S protein.
T81 2098-2174 Sentence denotes Viral genomic RNA is released and translated into viral polymerase proteins.
T82 2175-2468 Sentence denotes Viral RNA and nucleocapsid (N) structural protein are replicated, transcribed, or synthesized in the cytoplasm, whereas other viral structural proteins, including S, membrane (M), and envelope (E), are transcribed then translated in the endoplasmic reticulum (ER) and transported to the Golgi.
T83 2469-2649 Sentence denotes The viral RNA–N complex and S, M, and E proteins are further assembled in the ER–Golgi intermediate compartment (ERGIC) to form a mature virion, then released from host cells [62].
T84 2650-2822 Sentence denotes Natural products of HS and the allied polysaccharide, heparin, are involved and prevent infection by a range of viruses including S-associated coronavirus strain HSR1 [66].
T85 2823-2934 Sentence denotes HS is known to bind CoV surface proteins and to be used by coronavirus for its attachment to target cells [54].
T86 2935-3103 Sentence denotes Currently, there are no commercially available medicinal products designed to treat and/or prevent infections associated with the novel SARS-CoV-2 coronavirus outbreak.
T87 3104-3270 Sentence denotes The surface plasmon resonance and circular dichroism were used to measure the interaction between the SARS-CoV-2 Spike S1 protein RBD (SARS-CoV-2 S1 RBD) and heparin.
T88 3271-3365 Sentence denotes Additionally, basic amino acids are known to dictate the binding between proteins and heparin.
T89 3366-3634 Sentence denotes Primary sequence analysis of the expressed protein domain and analysis of the modeled SARS-CoV-2 S1 RBD structure show that there are several potential heparin binding sites, and more importantly, theses patches of basic amino acids are exposed on the protein surface.
T90 3635-3804 Sentence denotes This study has implications for the rapid development of a first-line therapeutic by repurposing heparin and for next-generation, tailor-made, GAG-based antivirals [66].