| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T861 |
0-37 |
Sentence |
denotes |
siRNA-based knockdown of host kinases |
| T862 |
38-64 |
Sentence |
denotes |
(Institut Pasteur, Paris). |
| T863 |
65-195 |
Sentence |
denotes |
Host kinases were knocked-down in A549 cells stably expressing ACE2 cells using OnTargetPlus siRNA SMARTpools (Horizon Discovery). |
| T864 |
196-299 |
Sentence |
denotes |
Briefly, A549-ACE2 cells seeded at 2x104 cells/well in 96-well plates 18 hours prior to the experiment. |
| T865 |
300-474 |
Sentence |
denotes |
The next day, each well was transfected with 6 pmoles of siRNA SMARTpool, using Lipofectamine RNAiMAX (Thermo Fisher Scientific) according to the manufacturer’s instructions. |
| T866 |
475-613 |
Sentence |
denotes |
Twenty-four (24) hours post transfection, the cell culture supernatant was removed and replaced with virus inoculum (MOI of 0.1 PFU/cell). |
| T867 |
614-774 |
Sentence |
denotes |
Following a 1 hour adsorption at 37°C, the virus inoculum was removed and replaced with fresh Opti-MEM media (Thermo Fisher Scientific) containing 2% FBS (v/v). |
| T868 |
775-946 |
Sentence |
denotes |
Seventy-two (72) hours post-infection (p.i.), the cell culture supernatant was heat-inactivated at 95°C for 5 minutes, and detection of viral genomes performed by RT-qPCR. |
