| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T745 |
0-123 |
Sentence |
denotes |
For each sample batch, 400 μL (30 μL per sample) of 50% Superflow bead slurry (QIAGEN) was added to a 2 mL bio-spin column. |
| T746 |
124-350 |
Sentence |
denotes |
Beads were incubated with 4 × 500 μL of 100 mM EDTA for 30 s, washed with 2 × 500 μL H2O, incubated 4 × 500 μL with 15 mM FeCl3 for 1 minute, washed 3 × 500 μL H2O, and washed once with 500 μL of 0.5% FA to remove residual Fe. |
| T747 |
351-499 |
Sentence |
denotes |
Beads were resuspended in 600 μL of H2O and 60 μL were aliquoted into a C18 NEST column that had been equilibrated with 150 μL of 80% ACN, 0.1% TFA. |
| T748 |
500-657 |
Sentence |
denotes |
1 mg of digested peptides were resuspended in 75% ACN/0.15% TFA and incubated with beads for 2 minutes, mixed by pipetting and incubated again for 2 minutes. |
| T749 |
658-866 |
Sentence |
denotes |
Beads were washed 4 × 200 μL with 80% ACN, 0.1% TFA, then washed 3 × 200 uL with 0.5% FA, incubated 2 × 200 μL with 500 mM potassium phosphate buffer pH 7 and incubated 2 × 200 μL with 0.5% FA for 15 seconds. |
| T750 |
867-975 |
Sentence |
denotes |
Phosphopeptides were eluted by centrifugation at 3000 RPM for 30 seconds with 2 × 75 uL of 50% ACN, 0.1% FA. |
