LitCovid-sentences  

PMC:7321036 / 76081-79167 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T692 0-38 Sentence denotes Experimental Model and Subject Details
T693 40-45 Sentence denotes Cells
T694 46-398 Sentence denotes African green monkey kidney epithelial Vero E6 (Vero 76, clone E6, Vero E6, ATCC® CRL-1586) authenticated by ATCC and tested negative for mycoplasma contamination prior to commencement were maintained in a humidified atmosphere at 37°C with 5% CO2, in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (v/v) fetal bovine serum (FBS, Invitrogen).
T695 399-499 Sentence denotes ACE2-expressing A549 cells, a human lung epithelial cell line, were a kind gift from Brad Rosenberg.
T696 500-599 Sentence denotes A549-ACE2 cells were cultured in DMEM supplemented with 10% FBS and maintained at 37°C with 5% CO2.
T697 600-747 Sentence denotes Caco-2 (ATCC® HTB-37) human colon epithelial cells were maintained in a humidified atmosphere at 37°C with 5% CO2 in DMEM-containing 20% (v/v) FBS.
T698 749-782 Sentence denotes Viruses (Institut Pasteur, Paris)
T699 783-964 Sentence denotes The SARS-CoV-2 isolate BetaCoV/France/IDF0372/2020 was supplied by the National Reference Centre for Respiratory Viruses hosted by Institut Pasteur (Paris, France) and headed by Pr.
T700 965-985 Sentence denotes Sylvie van der Werf.
T701 986-1044 Sentence denotes The isolate originated from a human sample provided by Dr.
T702 1045-1047 Sentence denotes X.
T703 1048-1063 Sentence denotes Lescure and Pr.
T704 1064-1066 Sentence denotes Y.
T705 1067-1119 Sentence denotes Yazdanpanah from the Bichat Hospital, Paris, France.
T706 1120-1208 Sentence denotes The isolate was supplied through the European Virus Archive goes Global (EVAg) platform.
T707 1209-1301 Sentence denotes Viral stocks were prepared by propagation in Vero E6 cells in DMEM supplemented with 2% FBS.
T708 1302-1442 Sentence denotes Viral titers were determined by plaque assay in Minimum Essential Media (MEM) supplemented with 2% (v/v) FBS (Invitrogen) and 0.05% agarose.
T709 1443-1633 Sentence denotes All experiments involving live SARS-CoV-2 were performed in compliance with Institut Pasteur Paris’s guidelines for Biosafety Level 3 (BSL-3) containment procedures in approved laboratories.
T710 1634-1716 Sentence denotes All experiments were performed in at least three biologically independent samples.
T711 1718-1749 Sentence denotes Viruses (Mount Sinai, New York)
T712 1750-2036 Sentence denotes For infection experiments in ACE2-A549 cells, SARS-CoV-2, isolate USA-WA1/2020 (NR-52281), which shares 99.983% sequence identity with the BetaCoV/France/IDF0372/2020 isolate, was deposited by the Center for Disease Control and Prevention and obtained through BEI Resources, NIAID, NIH.
T713 2037-2272 Sentence denotes SARS-CoV-2 was propagated in Vero E6 cells in DMEM supplemented with 2% FBS, 4.5 g/L D-glucose, 4 mM L-glutamine, 10 mM Non-Essential Amino Acids, 1 mM Sodium Pyruvate and 10 mM HEPES as described previously (Blanco-Melo et al., 2020).
T714 2273-2519 Sentence denotes All work involving live SARS-CoV-2 was performed in the CDC/USDA-approved BSL-3 facility of the Global Health and Emerging Pathogens Institute at the Icahn School of Medicine at Mount Sinai in accordance with institutional biosafety requirements.
T715 2521-2562 Sentence denotes Viruses (University of Freiburg, Germany)
T716 2563-2728 Sentence denotes For infection experiments in Caco-2 cells, SARS-CoV-2 isolate Muc-IMB-1/2020, kindly provided by the Bundeswehr Institute of Microbiology, Munich, Germany, was used.
T717 2729-2805 Sentence denotes SARS-CoV-2 was propagated in Vero E6 cells in DMEM supplemented with 2% FBS.
T718 2806-3086 Sentence denotes All work involving live SARS-CoV-2 was performed in the BSL-3 facility of the Institute of Virology, University Hospital Freiburg, and was approved according to the German Act of Genetic Engineering by the local authority (Regierungspraesidium Tuebingen, permit UNI.FRK.05.16/05).